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When should I pot up seedlings?

Soon after the first open, or when leaves from neighboring cells start to overlap and roots show at the drainage holes. Move tomatoes, peppers and eggplant into pots about 3 to 4 inches across. Squash, cucumbers and melons are better sown directly in their own pots and not moved until planting out.

Based on University of Maryland Extension and Purdue Extension · Updated Oct 2026

What to do now

Mildest step first.

  1. Look for the first true leaves, the ones that look like the plant's real leaves, above the round seed leaves.
  2. Water an hour ahead, then lift each seedling out with a butter knife or popsicle stick, holding it by a leaf, not the stem.
  3. Set it in a 3- to 4-inch pot of moist potting mix at the same depth; tomatoes can go deeper.
  4. Keep it out of strong light for a day or two, then put it back under lights 14–16 hours a day.

When they're ready

Seeds sown together in a flat or small cell need more room soon after their first true leaves appear. Those are the leaves that form after the round seed leaves (cotyledons) and look like a small version of the plant's real foliage. University of Maryland Extension calls that the ideal time to move them and says leaving seedlings in the seed flat too long is one of the most common mistakes; Purdue warns that waiting produces crowded, spindly plants. Clemson allows up to two sets of true leaves. Utah State uses two other signs: leaves starting to overlap with neighboring plants, and roots visible when you slide a plant out, before they form a dense mat.

What size pot

For tomatoes, peppers and eggplant started in small cells, the next step is a pot about 3 to 4 inches across; Maryland suggests cells or pots of 9 to 16 square inches for these crops. Bigger is not better at this stage: a large pot of wet mix around a small root ball stays soggy and invites root rot. Fast-growing tomatoes may need a second move into a larger pot if planting-out is still weeks away. Broccoli and cabbage do well in somewhat smaller cells, around 4 to 9 square inches.

How to move them

Water the flat an hour before so the mix holds together. Lift each seedling with a butter knife, chopstick or popsicle stick, keeping as much mix around the roots as you can, and tease crowded seedlings apart gently. Hold them by a leaf, not the stem: a crushed stem can't repair itself, but a torn leaf is soon replaced. Set the seedling in its new pot at the depth it grew before and firm the mix lightly. Tomatoes are the exception; they root along the buried stem, so you can set them deeper, up to just below the seed leaves.

Aftercare

Newly moved seedlings may droop for a day. Purdue suggests keeping them out of strong light for a few days; under fluorescent lights they usually do fine. Then return them to 14 to 16 hours of light a day. Fertilizer depends on your mix: Maryland notes that most potting mixes with added fertilizer carry enough for about 6 weeks, so wait. If your mix has none, water with a half-strength soluble fertilizer every week or two. Too much fertilizer makes soft, growth.

Crops to leave alone

Squash, cucumbers, melons and pumpkins don't tolerate root disturbance well. Colorado State says the only workable way to transplant them is to start seedlings in pots and move them without damaging the roots. Maryland suggests sowing 2 or 3 seeds per container, growing them for only 2 to 4 weeks, and skipping entirely. to one by snipping the extras with scissors rather than pulling them. Corn and beans are handled the same way, or sown directly in the garden.

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